Lyra Influenza A+B Assay

The real-time influenza test has been shown to detect HIN1, H3N2 and H7N9 subtypes but does not differentiate the subtypes from Influenza A.

A multiplex RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for each of the target viruses present in the sample. Identification of influenza A occurs by the use of target specific primers and a fluorescent-labeled probe that hybridizes to a conserved region within the matrix protein gene. Identification of influenza B occurs by the use of target specific primers and fluorescent-labeled probes that hybridize to a conserved influenza B sequence within the neuraminidase gene.

Test Type: rRT-PCR

Specimen Type*: Nasal and Nasopharyngeal swab specimens

Turnaround time ~2.5 hours (60 minutes for extraction and 75 minutes for amplification and detection)

CLIA complexity: Highly complex

FDA status: Approved

Validation Data :

Nucleic Acid Extraction:

• bioMerieux NucliSENS® easyMAG®
• bioMerieux EMAG®

Nucleic Acid Amplification and Detection:

• Applied Biosystems^® 7500 Fast Dx Real-Time PCR Instrument
• QuantStudio^TM Dx Real-Time PCR Instrument
• Cepheid^® SmartCycler^® II

*Viral transport media (M4, M4-RT, M5, M6, and UTM) are compatible with the Lyra respiratory assays